Disruption of URA-3 gene of a paclitaxel-producing fungus N8
10.3760/cma.j.issn.1673-4181.2014.05.001
- VernacularTitle:产紫杉醇真菌N8菌株URA-3基因的敲除
- Author:
Fei YAN
- Publication Type:Journal Article
- Keywords:
Paclitaxel-producing fungus;
URA-3;
Gene knock-out
- From:
International Journal of Biomedical Engineering
2014;37(5):257-261,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective In order to get genetic markers,an auxotrophic paclitaxel-producing fungus named Pestalotiopsis malicola N8 strain was isolated by genetic modification.Methods Based on the homologous recombination,URA-3 which is the key gene for uracil synthetic route of Pestalotiopsis malicola N8 strain was knocked out.The transformants were screened by minimal medium with the combination of 5-fluoroorotic acid (5-FOA) and uracil.Results The results showed that the uracil auxotrophic strain was able to grow in the minimal medium containing 5-FOA and uracil while the wild type strain was not.Conclusions The uracil auxotrophic strain can be used as a new selection marker for future gene function studies of N8 strain.
