Construction and identification of KIF18A protein baculovirus expression system
10.3760/cma.j.issn.1673-4181.2015.02.003
- VernacularTitle:KIF18A杆状病毒表达系统的构建与鉴定
- Author:
Zedong ZHAO
;
Chenghua FU
;
Shuangshuang LIANG
;
Changjun ZHU
;
Lina PAN
- Publication Type:Journal Article
- Keywords:
KIF18A protein;
Baculovirus;
Bac-to-Bac
- From:
International Journal of Biomedical Engineering
2015;38(2):73-76,后插4
- CountryChina
- Language:Chinese
-
Abstract:
Objective KIF18A is a protein that has close relation with mitotic regulation and tumor development.This study aimed to establish KIF18A protein expression system in baculovirus,which may help to realize high efficient synthesis of KIF1 8A protein in vitro.Methods Transfer vector was constructed with molecular cloning method,and recombinant baculovirus were obtained through gene transposition.KIF18A protein was expressed by transforming recombinant baculovirus into infected insect Sf-9 cells to realize the synthesis efficiency.Results It was confirmed by DNA sequencing,microscopic observation and Western Blot that the KIF18A protein expression system in baculovirus was successfully established.Conclusions Conditions for transfer vector and recombinant virus transfection are defined,and high efficient KIF18A protein baculovirus expression system are successfully constructed.
