Proliferation activating effects of Mtb-Ag on γδT cells and its effects on expression of CD69 molecules inγδT cells
10.3760/cma.j.issn.1673-4181.2015.01.007
- VernacularTitle:Mtb-Ag活化γδT细胞的扩增及对γδT细胞CD69分子表达的影响
- Author:
Hongmei JIN
;
Li ZHU
;
Keqiang WANG
;
Guo LIU
;
Yanchao DUAN
;
Zhenxin YUAN
- Publication Type:Journal Article
- Keywords:
γδT cells;
Mycobacterium tuberculosis low molecular peptide antigen;
CD69 molecules
- From:
International Journal of Biomedical Engineering
2015;38(1):29-31,35
- CountryChina
- Language:Chinese
-
Abstract:
Objective To activate and amplify γδT cells with low molecular peptide antigen of Mycobacterium tuberculosis low molecular peptide antigen (Mtb-Ag),and to investigate the expression of CD69 molecules on γδT cellular surface.Methods Healthy human peripheral blood mononuclear cells (PBMC) were obtained and separated,then positive cells were isolated by immuno-magnetic beads selection,and the proportion of γδT cells in the PBMCs was detected by fluorescent monoclonal TCR γδT-PE staining and flow cytometry.Expression of CD69 molecules in γδT cells was detected by γδ-PE/CD69 FITC double staining.Results The proportion of γδT cells was (4.9±1.85)％ in freshly obtained PBMC,(69.2±6.57)％ after 10 d of Mtb-Ag activation,and (99.3±8.92)％ after immuno-magnetic beads selection.The expression of CD69 molecules in γδT cells reached the peak (75.2％) at 24 h after initial Mtb-Ag stimulation,and reached the peak (72.0％) at 6 h after second stimulation.Conclusions MtbAg can specifically stimulate the proliferation of γδT cells in the PBMC.Both its initial and the second stimulation can specifically activate γδT cells.
