Study on tumor necrosis factor-α induced eryptosis
10.3760/cma.j.issn.1673-4181.2014.06.005
- VernacularTitle:肿瘤坏死因子-α诱导红细胞衰亡的研究
- Author:
Hongxiang LIU
;
Guoguang MA
;
Hongze ZHANG
;
Fei CHEN
;
Wanhua YANG
;
Bin SHI
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-α;
Erythrocyte;
Eryptosis
- From:
International Journal of Biomedical Engineering
2014;37(6):337-340,后插6,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of tumor necrosis factor-α (TNF-α) on eryptosis.Methods Erythrocytes isolated from mice were put under the treatment of TNF-α at the dose of 1ng/ml for 6,12,24,48 and 72 h,or at different concentrations of 0.1,1 and 10 ng/ml for 24 h.The forward scatter (FSC),phosphatidylserine (PS)exposure and ceramide formation were determined by flow cytometry.Results Compared to control group,the decrease of FSC ((81.5 ± 1.02)％ vs (87.6 ± 0.55)％,P＜0.05),the increasment of membrane PS exposure level and ceramide content ((5.5±1.07)％ vs (2.7±0.17)％,(2.1±0.23)％ vs (0.7±0.26)％,P＜0.01) were observed in erythrocyte under the treatment of TNF-α for 24 h with more obvious tendency over time.Conclusions TNF-α can trigger cell shrinkage,and promote PS exposure and ceramide formation on the membrane of erythrocyte.
