Effect of over-expressed macrophage migration Inhibitory factor on epithelial-mesenchymal transition in human cervical carcinoma SiHa cells
10.3760/cma.j.issn.1008-1372.2015.04.002
- VernacularTitle:过表达MIF对宫颈癌SiHa细胞上皮-间质转化的影响
- Author:
Suyu ZHANG
;
Suhui WU
;
Hui LI
- Publication Type:Journal Article
- Keywords:
Blotting,Western;
Macrophage migration-inhibitory factors/ME/PD;
Uterine cervical neoplasms/ME/PA/DT;
Cell transformation,neoplastic/DE;
Epithelial cells/DE/PA;
Stromal cells/DE/PA
- From:
Journal of Chinese Physician
2015;17(4):484-486,490
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of over-expressed macrophage migration inhibitory factor (MIF) on epithelial-mesenchymal transition (EMT) in human cervical carcinoma SiHa cells.Methods Recombinant eukaryotic expression plasmid liposome enhanced transfection of green fluorescent protein gene (pEGFP-N1)-MIF was constructed and then transfected into human cervical cancer SiHa cells.Experimental cells were classified into three groups (SiHa-pEGFP-N1-MIF,SiHa-pEGFP-N1,and SiHa).Western blot was used to detect the expression of MIF protein,and the expressions of EMT-related markers such as E-cadherin and vimentin in SiHa cells were determined before and after transfection.Results The eukaryotic expression vector pEGFP-N1-MIF significantly increased the expression of MIF protein in SiHa cells (P ＜ 0.05),and after overexpression of MIF gene in SiHa cells,the expression of E-cadherin protein in SiHa-pEGFP-N1-MIF group was significantly lower than that in control groups (P ＜0.05),while the expression of vimentin in SiHa-pEGFP-N1-MIF group was significantly higher than that in control groups (P ＜ 0.05).Conclusions Overexpression of MIF in cervical cancer SiHa cells can promote the EMT occurrence.
