NDRG1 suppresses TGF-β-induced epithelial-mesenchymal transition in lung cancer A549 cells
10.3760/cma.j.issn.1008-1372.2015.05.020
- VernacularTitle:过表达NDRG1对TGF-β诱导肺癌A549细胞上皮-间质转化的逆转作用
- Author:
Hao LIU
;
Ying SONG
;
Zhimin HE
- Publication Type:Journal Article
- Keywords:
Intercellular signaling peptides and proteins/PD/ME;
Transforming growth factor beta/AE;
Lung neoplasms/PA;
Cell transformation,neoplastic/DE;
Epithelial cells/DE/PA;
Stromal ceils/DE/PA
- From:
Journal of Chinese Physician
2015;17(5):710-713,718
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect and molecular mechanism of N-myc downstream regulated gene 1 (NDRG1) on transforming growth factor-beta (TGF-β)-induced epithelial-mesenchymal transition (EMT) in human lung cancer cells.Methods Lung cancer A549 cells were transfected with NDRG1 overexpressed vector,and then treated with 5 μg/L TGF-β1.The abilities of invasion were detected by Transwell assay.The expressions of NDRG1 mRNA and protein were analyzed by teal-time reverse transcription polymerase chain reaction (RT-PCR) were examined with Western blot.The expressions of EMT-associated markers E-cadherin and Vimentin,and EMT-associated signaling molecules Snail,AKT and Smad were detected with Western blot.Results We found that TGF-β1 treatment could induce morphological alteration of A549 cells from epithelial morphology to mesenchymal morphology.TGF-β1 significantly increased the migration of A549 cells,and increased the expression of mesenchymal maker vimentin and decreased epithelial marker E-cadherin.More importantly,overexpression of NDRG1 significandy reversed the effects of TGF-β1 on A549 cells.Moreover,NDRG1 significandy decreased the levels of phospho-AKT,and suppressed the expression of EMT-related transcription factor Snail.Conclusions NDRG1 could reverse the effects of TGF-β1 on EMT in A549 cells,by which mechanism is related to reduction of the expressions of phospho-AKT and Snail.
