Effect of miRNA-200b-specific inhibitor on biological characteristics of rat hepatic stellate cells
10.3760/cma.j.issn.1008-1372.2015.05.012
- VernacularTitle:特异性miRNA-200b抑制剂对肝星状细胞生物学特性的影响
- Author:
Rongquan FU
;
Jiguang DING
;
Liang HONG
;
Danping HU
;
Jinguo WU
- Publication Type:Journal Article
- Keywords:
MicroRNAs/AI/PD/ME;
Hepatocytes/CY/DE;
Astrocytes/DE;
Cell proliferation/DE;
Collagen/ME;
Actins/ME;
Hyaluronic acid/ME
- From:
Journal of Chinese Physician
2015;17(5):682-684,688
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miRNA-200b-specific inhibitor on hepatic stellate cells(HSCs) activation,proliferation,and extracellular matrix production.Methods The miRNA-200b-specific inhibitors were designed,synthesized,and transfected into HSCs with lipofectamine 2000.The supernatant and HSCs were collected after incubation for 48 h.The expression of miR-200b was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR).The expression ofα-smooth muscle actin (oα-SMA) protein in HSCs was detected by Western blotting.The cell proliferation was assayed by methyl thiazolyl tetrazolium (MTT) method.Contents of type Ⅲ procollagen and hyaluronic acid in supernatant were determined by radioimmunoassay.Results Compared to the control group,miRNA-200b expression was decreased in the miRNA-200b inhibitor group by 82％ (P ＜ 0.01),α-SMA protein expression was reduced in the miRNA-200b inhibitor group by (19 ± 3) ％ (P ＜ 0.05),and the activity of HSCs proliferation was reduced by(33 ± 5)％ (P ＜0.01),and the contents of type Ⅲ procollagen and hyaluronic acid in supernatant were reduced in miRNA-200b inhibitor group by (35 ± 4)％ and (31 ± 2)％,respectively(P ＜0.01).Conclusions The miRNA-200b-specific inhibitor could significantly reduce the expression of miRNA-200b,and inhibit HSC proliferation,activation,and extracellular matrix production.
