Effect of miR-218 on proliferation and cell cycle of gastric cancer cells
10.3760/cma.j.issn.1008-1372.2015.07.018
- VernacularTitle:miR-218对胃癌细胞增殖及周期影响的研究
- Author:
Meiyuan LIU
;
Xiaoye HU
;
Lei MA
;
Qingfeng ZOU
- Publication Type:Journal Article
- Keywords:
MicroRNAs/PD/ME;
Stomach neoplasms/DT/ME;
Cell proliferation/DE;
Cell cycle/DE
- From:
Journal of Chinese Physician
2015;17(7):1017-1018,1023
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of miR-218 on cell proliferation and cell cycle in the gastric cancers (GC).Methods SGC-7901 and MGC-803 cells were transfected with miR-218 mimics.Meanwhile,SGC-7901 and MGC-803 cells were transfected with control mimics (Scramble mimics) as negative control.Quantitative reverse transcription polymerase chain reaction (qRT-PCR) was used to detect the miR-218 expression in these transfected cells.The effect of miR-218 overexpression on cell proliferation was evaluated with direct cell counting and MTS [3-(4,5-dimethylthiazol-2-yl)-5 (3-carboxymethonyphenol)-2-(4-sulfophenyl)-2H-tetrazolium inner salt] assay.Moreover,the effect of miR-218 overexpression on cell cycle was examined by fluorescence activated cell sorting (FACS).Results miR-218 expression was remarkably induced in miR-218-transfected cells,miR-218 overexpression led to a significant decrease in cell proliferation rate compared to control cells (P ＜ 0.05).miR-218 overexpression induced GC G1 arrest.Conclusions miR-218 can suppress GC cell proliferation and block cell cycle progression.It maybe play an important role in tumorigenesis and development of GC.
