Expression of cancer stem cell markers, CD133, CD44 and OCT-4, in small cell lung cancer and their clinical significances

VernacularTitle:肿瘤干细胞标志物CD133、CD44、OCT-4在小细胞肺癌中的表达及其临床意义
Author: Yajun JI ; Ling BAI ; Xin WANG ; Zhenghong LIU
Publication Type:Journal Article
Keywords: CD133; CD44; OCT-4; Cancer stem cell; Small cell lung cancer
From: Cancer Research and Clinic 2015;(7):458-460,464
CountryChina
Language:Chinese
Abstract: Objective To determine the expression of cancer stem cell markers, CD133, CD44 and OCT-4, in small cell lung cancer cell line NCI-H82 and confirm the specific markers. Methods NCI-H82 cells were cultured, and the expressions of markers (CD133, CD44 and OCT-4) were detected by immunofluorescence staining. The immunohistochemistry was performed to detect the expressions of CD133, CD44 and OCT-4 in 79 tissues with small cell lung cancer. Results In NCI-H82 cells, the florescence was positive in CD133 and CD44 and negative in OCT-4. In the tissues, the expression of CD133 and CD 44 was related with tumor size, lymph node metastasis and clinical stage (P< 0.05), while OCT-4 was negative expression. Conclusion CD133 and CD44 might be the cancer stem cell markers of small cell lung cancer, which maybe have clinical significance on diagnosis and treatment.