Effect of Jinfeng particles on MMP-1/TIMP-1 expression in articular cartilage of gouty arthritis in rabbits
10.3969/j.issn.1000-484X.2015.06.012
- VernacularTitle:金凤颗粒对痛风性关节炎兔关节软骨MMP-1/TIMP-1表达影响
- Author:
Xiaoqian WANG
;
Xin LI
;
Jiansheng GUO
;
Kejian ZHU
- Publication Type:Journal Article
- Keywords:
Jinfeng particles;
Gouty arthritis;
MMP-1/TIMP-1
- From:
Chinese Journal of Immunology
2015;(6):774-777,784
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the effect of Jinfeng particles on the expression of MMP-1/TIMP-1 in cartilage of gout arthritis rabbit′s articular,to explicit treatment mechanism of gouty arthritis. Methods:Perfusion of ethambutol and adenine,inject MSU into joint cavity of rabbits to copy the model of gouty arthritis. After successful mode,were randomly divided into model group,Jinfeng granules in high,medium and low dose group(19. 6,9. 8,4. 9 g/kg),allopurinol group(0. 028 g/kg),Tongfengding group(0. 448 g/kg) ,another blank group. Administered for 7 days. 2 h after the last administration,were taken and painted articular cartilage tissue,to observe the changes of the pathological morphology;and the use of immune cell detection of the expression of TIMP-1,MMP-1,MMP-1/TIMP-1 ratio calculation. Results: Jinfeng granule could obviously reduce the articular cartilage of rats with inflammatory cells infiltration,hyperemia, swelling and necrosis, could significantly inhibit the expression of MMP-1 ( P< 0. 05 ) , stimulate TIMP-1 activation(P< 0. 01),and significantly reduce the ratio of MMP-1/TIMP-1(P< 0. 05). Conclusion: Jinfeng particles can effectively inhibit the pathological damage of gouty arthritis,upside the expression of TIMP-1 and its mechanism may be related to the inhibition of MMP-1,regulated the balance of MMP-1/TIMP-1,and then exert anti-inflammatory effects.
