Dynamic changes of IL-27, IL-10, IL-17 in serum of collagen-induced arthritis in mice
10.3969/j.issn.1000-484X.2015.06.018
- VernacularTitle:CIA小鼠血清中IL-27、IL-10、IL-17的动态分析
- Author:
Yachun GUO
;
Guiying FENG
;
Hongru SONG
;
Enhong XING
;
Gao AN
;
Xiaofei ZHAO
- Publication Type:Journal Article
- Keywords:
Collagen-induced arthritis;
IL-17;
IL-27;
IL-10;
Flow cytometry
- From:
Chinese Journal of Immunology
2015;(6):803-805,821
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe chicken typeⅡcollagen-induced arthritis( CIA) in serum of mice with the dynamic changes of IL-27,IL-17,IL-10. Methods: 54 DBA1/J mice were randomly divided into control group(n=6) and model group(n=12), according to the progress of CIA mouse course early,middle and late(7,14,and 35 days after booster immunization) ,taking the eyeball for blood and separating the serum under sterile condition. The dynamic levels of cytokines IL-27,IL-17,IL-10 were detected by flow cy-tometry. Results:The level of IL-27 in the model group was significantly declined in the the progress of CIA mouse course early and middle(P<0. 05,P<0. 01) compared with the control group,but there was no significant difference between late disease and control group(P> 0. 05);the level of IL-17 in the disease early and late was no significant difference(P> 0. 05) compared with the control group,while in the mid course significantly higher than control group(P<0. 05) during the course of disease. The level of IL-10 showed no difference from the experiment beginning(P> 0. 05). Conclusion: IL-27,IL-17,IL-10 paticipate in the pathogenesis of CIA and their alterations at different stages of the disease have a relation to the development of arthritis.
