Histamine H3 receptor inhibited electrically evoked cytoplasmic calcium in differentiated skeletal C2C12 myoblasts
10.3969/j.issn.1000-4718.2015.06.026
- VernacularTitle:组胺 H3受体降低电激发收缩的小鼠成肌细胞胞浆中钙离子浓度
- Author:
Lin QI
;
Xiao FENG
;
Yan CHEN
;
Rui XUE
;
Feng ZHANG
;
Suyun WANG
;
Suke SUN
;
Jianguo WEN
- Publication Type:Journal Article
- Keywords:
Myogenesis;
Histamine H3 receptor;
Electrical stimulation;
Calcium ion
- From:
Chinese Journal of Pathophysiology
2015;(6):1115-1119,1124
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the expression and possible function of histamine H3 receptor (H3R) in striated myogenesis and the differentiated C2C12 cells.METHODS: H3R and myogenesis late marker myosin heavy chain (MHC) were detected at mRNA and protein levels during C2C12 myogenesis.H3R antagonist ciproxifan was added and the expression of the myogenesis early marker desmin, intermediate markers myogenin and MHC was detected.Differentia-ted myoblasts were loaded with Fluo-4 calcium indicator dye and the effect of R-( a)-methylhistamine ( RMeHA) on the cy-toplasmic calcium concentration was determined under the 200 mA electrical stimulation.RESULTS: The expression of H3R and MHC was increased during myogenesis.Ciproxifan incubation had no influence on the 3 striated myogenesis mar-kers (P>0.05).In C2C12 myoblasts, RMeHA (10 nmol/L~100 μmol/L) effectively diminished cytoplasmic calcium peak when the cells were electrically paced (P<0.05).The best inhibitory effect of RMeHA was observed at dose of 100 nM for 10 min and 20 min, which was higher than that for 5 min (P<0.05).CONCLUSION: H3R might have little effect on the myogenic differentiation, but diminishes cytoplasmic calcium peak of the differentiated myoblasts under electri-cal stimulation.
