Protective effect of ecdysterone on rabbits chondrocytes that is injured by lipopolysaccharide
10.11958/j.issn.0253-9896.2015.06.003
- VernacularTitle:蜕皮甾酮对脂多糖诱导兔软骨细胞损伤的保护作用
- Author:
Gangtao WANG
;
Xuhui ZHANG
;
Weidong ZHANG
;
Lei XIA
- Publication Type:Journal Article
- Keywords:
Ecdysterone;
chondrocytes;
lipopolysaccharides;
cell proliferation;
apoptosis;
interleukin-1beta;
iNOS
- From:
Tianjin Medical Journal
2015;(6):587-589,590
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of ecdysterone (EDS) on rabbits chondrocytes that is injuried by lipopolysac?charide (LPS). Methods Aricular chondrocytes were isolated from rabbits and randomly divided into three groups:control group;chondrocytes with LPS induced injury (LPS group);injury chondrocytes treated with EDS (LPS+EDS group). The cell proliferation and cell apoptosis of chondrocytes were determined by MTT method and flow cytometry assay respectively. The mRNA and protein expression levels of inducible nitric oxide synthase (iNOS) were detected by RT-PCR and western blot. In addition, the content of NO and IL-1βwere measured by nitric acid reductase assay and enzyme-linked immunosorbent assay (ELISA) respectively. Results Attenuated proliferation, increased cell apoptosis, iNOS, NO and IL-1βwere seen in LPS group , but all these changes were significantly reversed by EDS treatment (P<0.05). Conclusion Ecdysterone exhib?ited a protective effect on LPS induced rabbits chondrocytes injury through inhibiting the expression of iNOS.
