In vitro growth characteristics of bone marrow mesenchymal stem cells derived from patients with multiple myeloma and significance of TGF-β1 gene expression

VernacularTitle:多发性骨髓瘤患者骨髓间充质干细胞体外生长特性和转化生长因子-β1基因的表达及其意义
Author: Yanhua LEI ; Yilong LU ; Wei ZHU ; Fang LEI ; Xianqiu YU ; Xiaoming FEI ; Yan ZHU
Publication Type:Journal Article
Keywords: Multiple myeloma; Bone marrow mesenchymal stem cells; Transforming growth factor-β1
From: Journal of Leukemia & Lymphoma 2013;22(4):220-222,225
CountryChina
Language:Chinese
Abstract: Objective To investigate the expression and significance of transforming growth factor-β1 (TGF-β1) gene in bone marrow mesenchymal stem cell (BMMSC) derived from patients with multiple myeloma (MM).Methods BMMSC of 7 MM patients and 10 patients with iron deficiency anemia were cultured in vitro.The morphology of BMMSC was observed and the growth curve was portrayed according to the daily results of BMMSC proliferation.Total RNA was extracted from BMMSCs and transcription of TGF-β1 gene in BMMSC was measured by reverse transcription-PCR.Results The proliferative activity of BMMSC was not significantly different between the two groups,but expression of TGF-β1 gene of BMMSC was higher in MM patients (0.01241±0.00419) than the control group (0.00122±0.00030) (t =3.218,P ＜ 0.05).Conclusion The abnormally high expression of TGF-β31 gene in BMMSCs could contribute to the pathogenesis of MM.