Effect of matrix metalloproteinase-9 on the formation of soluble MICA protein in osteosarcoma cells
10.3969/j.issn.1006-5725.2015.11.004
- VernacularTitle:基质金属蛋白酶－9对骨肉瘤细胞可溶性MICA蛋白形成的影响
- Author:
Shanming LU
;
Ling XUE
;
Wangdong DENG
- Publication Type:Journal Article
- Keywords:
Osteosarcoma;
MHC class I chain-related A;
Matrix metalloproteinase-9;
Soluble MICA
- From:
The Journal of Practical Medicine
2015;(11):1741-1743,1744
- CountryChina
- Language:Chinese
-
Abstract:
Objective To provide a new putative target for immunotherapy on osteosarcoma and explore the effect of matrix metalloproteinase-9 (MMP-9) on the formation of soluble MICA protein in osteosarcoma cells. Methods MMP-9 antisense oligonucleotide was transfected to osteosarcoma cells with lipofectamine 2000. MMP-9 mRNA was assessed by RT-PCR. MMP-9 activity and soluble MICA (sMICA) in the culture supernatant was examined by zymography and quantified by ELISA, respectively. Results MMP-9 mRNA expression and gelatin enzymatic activity were inhibited in MMP-9 antisense oligonucleotide group. Comparing with the control group, lower concentration of sMICA was found in MMP-9 antisense oligonucleotide group (P = 0.011). Conclusion MMP-9 may have an effect on the formation of sMICA protein in osteosarcoma. MMP-9 could be regarded as a putative target for immunotherapy in osteosarcoma.
