The protective effect of mesenteric lymph duct ligation on systemic inflammatory responsein rats with severe acute pancreatitis
10.3760/cma.j.issn.1008-6706.2015.15.007
- VernacularTitle:肠系膜淋巴管结扎对重症急性胰腺炎大鼠全身炎性反应的保护作用
- Author:
Haiyan LIU
- Publication Type:Journal Article
- Keywords:
Pancreatitis;
Gytokines;
Systemic inflammatory response syndrome
- From:
Chinese Journal of Primary Medicine and Pharmacy
2015;(15):2260-2262,2263
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the role of mesenteric lymphatic pathway in severe acute pancreatitis (SAP)by detection of inflammatory factors levels and mesenteric lymph duct ligation in rats.Methods Twenty-four SD male rats were randomly divided into group A (SAP group),group B (SAP + mesenteric lymph duct ligation group)and group C(sham operation group),with eight rats in each group.Samples of portal vein blood and mesenter-ic lymph were collected for measuring the level of tumor necrosis factor α(TNF-α),interleukin 1β(IL-1β),interleu-kin 6(IL-6)and endotoxin at twelfth hour.The blood CDl b was tested at sixth hour.24h lactulose /mannitol excretion ratios(L/M)were taken as the intestinal mucosal permeability index by gavage of 100mg lactulose and 50mg mannitol solution.The terminal ileums were collected for pathological examination.Results The blood levels of endotoxin and cytokines(TNF-α,IL-1β,IL-6)were significantly increased in group A compared with group C(all P <0.01).Each factor′s concentration in lymph was much higher than that in portal vein blood,especially in group A.The endotoxin and cytokines(TNF-α,IL-1β,IL-6)levels in portal vein blood of group B were significantly decreased compared with group A(all P <0.05),but still higher than that of group C(all P <0.05).CDl b expression in group A was the highest,followed by group B,group C was the lowest (P <0.01).24h L/M in group A was significantly higher than that in group C (P <0.01),and L/Min group B decreased significantly compared with those in A group(P <0.05). Conclusion Lymphatic pathway is an important pathway of systemic inflammatory response induced by SAP,and ligation of mesenteric lymph duct has a protective effect on the organism.
