Comparison of Invariant NKT Cells with Conventional T Cells by Using Gene Set Enrichment Analysis (GSEA).
- Author:
Sae Jin OH
1
;
Ji Ye AHN
;
Doo Hyun CHUNG
Author Information
- Publication Type:Original Article
- Keywords: NKT cells; conventional T cells; Microarray; GSEA
- MeSH: Adaptive Immunity; Cytokines; Gene Expression; Ligation; Mental Competency; Natural Killer T-Cells; Receptors, Antigen, T-Cell; T-Lymphocytes
- From:Immune Network 2011;11(6):406-411
- CountryRepublic of Korea
- Language:English
- Abstract: BACKGROUND: Invariant Natural killer T (iNKT) cells, a distinct subset of CD1d-restricted T cells with invariant Valphabeta TCR, functionally bridge innate and adaptive immunity. While iNKT cells share features with conventional T cells in some functional aspects, they simultaneously produce large amount of Th1 and Th2 cytokines upon T-cell receptor (TCR) ligation. However, gene expression pattern in two types of cells has not been well characterized. METHODS: we performed comparative microarray analyses of gene expression in murine iNKT cells and conventional CD4+CD25-gammadeltaTCR- T cells by using Gene Set Enrichment Analysis (GSEA) method. RESULTS: Here, we describe profound differences in gene expression pattern between iNKT cells and conventional CD4+CD25-gammadeltaTCR- T cells. CONCLUSION: Our results provide new insights into the functional competence of iNKT cells and a better understanding of their various roles during immune responses.
