Th17 Responses Are Not Induced in Dextran Sodium Sulfate Model of Acute Colitis.
- Author:
Yoon Suk KIM
1
;
Min Ho LEE
;
Ahn Seung JU
;
Ki Jong RHEE
Author Information
- Publication Type:Brief Communication
- Keywords: Dextran sodium sulfate; Inflammatory bowel disease; Th17 cell; Colitis
- MeSH: Animals; Colitis; Colon; Dextrans; Flow Cytometry; Inflammatory Bowel Diseases; Interleukin-17; Interleukin-4; Mice; Models, Chemical; Sodium; Sulfates; T-Lymphocytes; Th1 Cells; Th17 Cells
- From:Immune Network 2011;11(6):416-419
- CountryRepublic of Korea
- Language:English
- Abstract: Dextran sodium sulfate (DSS) is a widely used chemical model for inflammatory bowel disease (IBD). It is thought that imbalances in the T helper (Th) cell subsets contribute to IBD. Recent studies suggest that the acute DSS-colitis model is polarized toward a Th1/Th17 profile based on RT-PCR analysis of colonic tissues. In the current study we determined whether colonic Th cells from DSS-colitis mice were skewed toward the Th17 profile. Mice were treated with 5% DSS for 7 days and colonic T cells isolated and examined for production of IFN-gamma (Th1 cell), IL-4 (Th2 cell) and IL-17 (Th17 cell) by intracellular flow cytometry. We found that the percentage of colonic Th17 cells were similar to non-treated controls but the percentage of Th1 cells were elevated in DSS-colitis mice. These results suggest that in the acute DSS-colitis model the colonic Th cells exhibit a Th1 profile and not a Th17 profile.
