Macrophage migration inhibitory factor potentially enhance the proliferation of fibroblast induced by lipopolysaccharide through increasing Toll-like recetor 4
10.3760/cma.j.issn.1007-7480.2014.11.011
- VernacularTitle:巨噬细胞移动抑制因子通过上调Toll样受体-4潜在性加强脂多糖诱导的成纤维细胞增殖
- Author:
Changyi XIE
;
Yebin XI
;
Zhengde XI
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Toll-like recetor 4;
Fibroblasts;
Cell proliferation;
Macrophage migration inhibitory factor
- From:
Chinese Journal of Rheumatology
2014;18(11):766-769,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective Based on the research of the mechanism of fibroblast proliferation,the principle of synovial hyperplasia is explored in rheumatoid arthritis (RA).Methods CCK-8 method was used to observe the stimulation effect of LPS on fibroblast proliferation.By confocal scanning microscopy,the expression of TLR-4 on fibroblast membrane was observed; Real-time fluorescent quantitative PCR method was used to detect the stimulatory effect of macrophage migration inhibitory factor on TLR-4 expression of fibrob-lasts and further analyzed the changes of TLR-4 expression on the cell membrane by flow cytometry.Results The LPS could stimulate fibroblast proliferation,and the fibroblasts expression of TLR4 per se.After stimulated by macrophage migration inhibitory factor,the expression of fibroblast TLR-4 was increased by about 20％-30％.Conclusion MIF increases the TLR-4 expression of fibroblasts that potentially enhance cell proliferation induced by LPS.
