Diagnostic value of flow cytometry in aggressive natural killer-cell leukemia: a case report and review of literatures

VernacularTitle:流式细胞术在侵袭性NK细胞白血病诊断中的价值:一例报道并文献复习
Author: Ying PAN ; Huiping WANG ; Cui ZHANG ; Qianshan TAO ; Dongdong YANG ; Shudao XIONG ; Zhimin ZHAI
Publication Type:Journal Article
Keywords: Flow cytometry; Aggressive; Natural killer-cell leukemia; Immunophenotype
From: Journal of Leukemia & Lymphoma 2014;23(1):38-40,44
CountryChina
Language:Chinese
Abstract: Objective To explore clinical characteristics and diagnosis of aggressive natural killercell leukemia (ANKL),and evaluate the value of flow cytometry (FCM) in diagnosing it.Methods A case of ANKL was reported and literatures were reviewed.Results The patient presented with persistent high fever,progressive pancytopenia and hepatosplenomegaly.The untypical cells could be seen by morphology.By FCM,NK cells consisted 83.3 ％ of total lymphocytes in bone marrow and immunophenotypes were CD34-,CD2+,CD7+,CD3-,CyCD3+,CD5-,CD16+,CD56+,CD30-,CD4-,CD8-,CD117-,CD11c,CD19-,CD45++,SSC+-++.T-cell receptor (TCR) and IgH gene rearrangement were negative and chromosome was normal.The patient was diagonised ANKL eventually.Conclusions ANKL is a quite rare disease with highly aggressive,poor prognosis and could be misdiagnosed easily.FCM combined with morphology is a convenient,handy,practicable and less invasive device for the diagnosis,and can be a preferred detection technique in some cases.