siRNA against survivin mediated by adenovirus vector up-regulate estrogen receptor expression in breast cancer MCF-7 cell
10.3760/cma.j.issn.1673-4203.2015.03.007
- VernacularTitle:腺病毒介导的靶向survivin的siRNA上调乳腺癌MCF-7细胞雌激素受体表达
- Author:
Haitao GUAN
;
Zhijun DAI
;
Zhongwei WANG
;
Xiaoxu LIU
;
Xijing WANG
- Publication Type:Journal Article
- Keywords:
Breast neoplasms;
Estrogen receptor alpha;
Survivin protein,human;
RNA,small interfering
- From:
International Journal of Surgery
2015;42(3):164-167,封3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect on ER expression in MCF-7 cell by siRNA against survivin mediated by adenovirus vector.Methods An adenovirus vector of siRNA against survivin was constructed and used to infect MCF-7 cell.The change of expression of survivin and ER was detected by Western Blot.Results The expression strength of survivin are 0.09 ± 0.04、0.86 ± 0.08、0.82 ± 0.17;expression strength of ER are 1.57 ± 0.09、1.16 ± 0.10、1.23 ± 0.01 respectively in the experimental group,negative control group and blank control group.Statistics analysis shows that the adenovirus vector of siRNA against survivin constructed in the study can suppress the expression of survivin significantly,and suppress the expression of survivin can up-regulate the estrogen receptor (ER) expression.Conclusions The results suggest that there may be a certain regulatory mechanism between survivin and ER signal pathway in MCF-7 cell and siRNA against survivin is of important potential value in the endocrine therapy of hormone receptor positive breast cancer.
