Establishment of an enzyme-linked immunosorbent assay for detecting of fumigate hydratase antibody and evaluation of its value in the diagnosis of autoimmune hepatitis
10.3760/cma.j.issn.1007-7480.2014.12.007
- VernacularTitle:抗延胡索酸水合酶抗体间接酶联免疫吸附试验法的建立及其对自身免疫性肝炎的诊断价值
- Author:
Huiyu LIAO
;
Yanli LI
;
Huiping YAN
;
Heqiu ZHANG
;
Qing XIA
;
Yan ZHAO
;
Haiping ZHANG
- Publication Type:Journal Article
- Keywords:
Hepatitis,autoimmune;
Fumarate hydratase;
Enzyme-linked immunosorbent assay
- From:
Chinese Journal of Rheumatology
2014;18(12):819-822,后插1
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the enzyme-linked immunosorbent assay (ELISA) for detecting human serum fumarate hydratase (FH) antibody and evaluate its role in the diagnosis of autoantigen in autoimmune hepatitis (AIH).Methods The indirect ELISA was established using FH protein,and the reaction conditions were determined.Then,the anti-FH antibody were detected in the serum of 88 AIH patients,56 primary biliary cirrhosis (PBC) patients,50 chronic hepatitis B (HBV) patients,36 chronic hepatitis C (HCV) patients and 98 healthy controls(HC).The results were analyzed with chi-quare and Kruskal-Wallis H methods.Results The ELISA for detecting human anti-FH antibody was established successfully and the optimal reaction conditions were defined.The positive rate of anti-FH antibody in the AIH group (40％) was significantly higher than HC (3％,x2=38.44,P＜0.01),PBC group (7％,x2=18.45,P＜0.01),CHB group (2％,x2=23.59,P＜0.01) and CHC group (6％,x2=14.29,P＜0.01).Anti-FH antibody which was used to diagnose AIH revealed a sensitivity of 40％ and specificity of 94％.Conclusion We have established the ELISA,which is used to detect human anti-FH antibody.It can be detected predominantly in AIH,and this implies that anti-FH antibody may be useful in improving the diagnosis of AIH.
