Mutation of Notch1 gene and its protein expression in T-cell lymphoma

VernacularTitle:T细胞淋巴瘤中Notch1基因突变及其蛋白表达的研究
Author: Xiaoxia WANG ; Shaoling WU ; Xindong ZHAO ; Xuemei WANG ; Zhan SU ; Can CHANG
Publication Type:Journal Article
Keywords: Lymphoma,T-cell; Mutation; Notch1 gene; Notch1 protein
From: Journal of Leukemia & Lymphoma 2013;22(4):212-214,219
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of the expression of Notch1 protein and the mutation of Notch1 gene in.T-cell lymphoma (TCL).Methods Immunohistochemistry was used to detect the expression of Notch1 protein,and PCR amplification and DNA sequencing were used to detect the mutation of Notch1 gene in the 26th and 27th HD domain and the 34th PEST domain in 30 cases.10 cases of reactive hyperplasia tissues of lymph node were as the control.Results The positive rates of Notch1 protein expression and Notch1 gene mutation were 70.0 ％ (21/30) and 56.7 ％ (17/30).8 cases of Notch1 mutations were detected in the HD domain,6 cases in the PEST domain,and 3 case in both HD and PEST domains.Inscrtion,deletion,nonsense mutation and missense mutation were included in Notch 1 mutations.Conclusion Notch1 gene mutation may play an important role in the expression of Notch1 protein.The occur of TCL is related to the expression of Notch1 protein and the mutation of Notch1 gene.