Expression of miR-9 in H/RS cells and its regulation on target PRDM1
10.3760/cma.j.issn.1009-9921.2013.05.002
- VernacularTitle:miR-9在H/RS细胞的表达及其对靶点PRDM1的调控
- Author:
Xinhua ZHOU
;
Xueping HUANG
;
Xinzhen DAI
;
Juan GE
;
Tong ZHAO
- Publication Type:Journal Article
- Keywords:
Lymphoma,Hodgkin;
miR-9;
PRDM1;
H/RS cell;
Redifferentiation
- From:
Journal of Leukemia & Lymphoma
2013;22(5):259-262,271
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the expression of miR-9 in H/RS cells and its regulation on target PRDM1.Methods miR-9 expression in normal CD19+ B-cell subsets and eight lymphoma cell lines was detected by fluorescence quantitative RT-PCR and in situ hybridization (ISH),for quantification and location,respectively.Chemically synthesizcd antisense oligonucleotide of miR-9 was transiently transfected into L428 for its silence,and the PRDM1 expression was tested.Results Fluorescence quantitative RT-PCR showed that the expression of miR-9 in L428 cells was marked higher than that of normal CD19+ B-cell subsets and other lymphoma cell lines (the expression of miR-9 in L428 cells was 47-fold of OCI-Ly1,50-fold of Raji cells,7-fold of EBV+ immortalized B cell line,and 6-fold of ALCL cell line).ISH indicated that miR-9 located in cytoplasm,it was a diffuse and strong positive in L428,scattered and weak in DLBCL and Burkitt' s lymphoma cell lines,while negative in KARPAS-299 or Jurkat cell lines.Transient down-regulation of miR-9 in L428 leded to the increase of PRDMI protein.Conclusion miR-9 plays the role of cancer gene in cHL,and may exert a potential function in regulating terminal B cell differentiation through a post transcription regulation of PRDM1 gene.
