Dose-response of S100A4 gene expression in human lymphocytes at different time points after irradiation

VernacularTitle:人淋巴细胞S100A4基因在照射后不同时间点表达水平的剂量-效应关系
Author: Jiangang FENG ; Shuang LI ; Jiangbin FENG ; Ling GAO ; Xue LU ; Deqing CHEN ; Qingjie LIU
Publication Type:Journal Article
Keywords: Real-time PCR; S100A4; Gene expression; 60Co γ-rays
From: Chinese Journal of Radiological Medicine and Protection 2015;35(4):245-247,302
CountryChina
Language:Chinese
Abstract: Objective To investigate the dose response of S100A4 gene expression in the irradiated lymphoblastoid cells AHH-1 at different time points post irradiation.Methods AHH-1 cells was exposed to different doses(0,1,3,5,8,10,15 and 18 Gy)of 60Co γ-rays,and its mRNA levels of S100A4 was detected by reverse transcription PCR and real-time PCR at 4,8,12,24,48 and 72 h after irradiation.Results Within the range of applied doses,the level of S100A4 gene expression was upregulated with a good dose-response (R2 =0.79-0.93,P ＜ 0.05) and had obvious difference at different time points (F =8.91,P ＜ 0.01).Conclusion S100A4 gene expression at transcriptional level could be detected easily and had optimum dose-responses at certain time points after irradiation,and hence is applicable as a dosimeter.