Research of Act1 in the pathogenesis in rheumatoid arthritis
10.3760/cma.j.issn.1007-7480.2015.02.009
- VernacularTitle:核因子κB激活剂1在类风湿关节炎发病机制中的研究
- Author:
Xiaohui ZHANG
;
Wei ZHOU
;
Juan ZHAO
;
Juan GUO
;
Zhuoli ZHANG
- Publication Type:Journal Article
- Keywords:
Arthritis,rheumatoid;
Interleukin-17;
Nuclear factor-kappa-B activator 1
- From:
Chinese Journal of Rheumatology
2015;19(2):110-114,后插2
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the nuclear factor-kappa-B activator 1 (Act1) expression in synovial tissues and peripheral blood lymphocytes in patients with rheumatoid arthritis (RA),and explore its possible regulatory mechanisms.Methods The Act1 expression of synovial tissue from 10 RA patients and 5 osteoarthritis patients were analyzed by immunohistochemical staining,and double immunofluorescence staining was used to identify the Act1-expressing cells.The peripheral blood of 47 RA patients and 22 healthy controls (HC) were obtained.The Act1 expression in different lymphocyte subpopulation from peripheral blood was determined by flow cytometry and Act1-mRNA expression was detected by real-time quantitative polymerase chain reaction (RT-PCR).The data was analyzed by Mann-Whitney U test,two independent sample t test and Pearson correlation analysis.Results Remarkable infiltration of inflammatory cells and expression of Act1 in synovium from RA patients was observed.The Act1 was expressed in T lymphocytes,in contrast to the few expression in B lymphocytes.Compared to HC,the expression of Act1-mRNA was lower in peripheral CD4+ T cells and CD8+ T cells [CD4+ T cells:133 (69,380) vs 158 (117,246),U=36,P＞0.05; CD8+ T cells:127(63,363) vs 129(81,222),U=35,P＞0.05],however,higher in CD20+ B cells in RA patients [62(34,81) vs 52(33,105),U =36,P ＞0.05].There was no significant difference of Act1-mRNA levels in peripheral blood mononuclear cells between HC and RA patients (0.71 ±0.32 vs 0.79±0.21,t=1.717,P=0.091).However,a tendency of down-regulation of Act1 expression was noticed in RA patients than HC.No significant correlation was found between Act1-mRNA level and RA disease activity index.Conclusion Our data suggest that Act1 may be involved in the pathogenesis of RA,however,we could not yet found its association with RA disease activity.
