Acute blood glucose fluctuation affects GSH-Px, IL-6, and NF-κB in aorta
10.3760/cma.j.issn.1008-1372.2015.01.020
- VernacularTitle:GSH-Px、IL-6及NF-κB在急性血糖波动的大鼠主动脉中的变化
- Author:
Wei ZHANG
;
Sheng ZHAO
;
Yan LI
;
Pin HAN
- Publication Type:Journal Article
- Keywords:
Glutathione peroxidase/metabolism;
Interleukin-6/metabolism;
NF-kappa B/metabolism;
Blood glucose;
Aorta
- From:
Journal of Chinese Physician
2015;17(1):71-73,77
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of acute blood glucose on the level of glutathione peroxidase (GSH-Px),interleukin-6 (IL-6),and the expression of nuclear factor-κB (NF-κB).Methods The Wistar rats were infused intermittently or consistently with 50％ glucose solution.The level of GSH-Px was investigated with colorimetry.The level of IL-6 was measured with enzyme-linked immunosorbent assay (ELISA).The expression of NF-κB was investigated with immunohistochemisty.Results The level of GSH-Px in aorta homogenate in the acute blood glucose fluctuation group [(6.26 ± 0.38) nmol/mgprot] was evidently lower than that of consistently high blood glucose group [(8.98 ± 0.56) nmol/mgprot] and the control group [(10.02 ±1.10)nmol/mgprot] (P ＜0.05).The level of IL-6 [(20.56 ±3.78)pg/ ml] and the expression of NF-κB in the aorta in the acute blood glucose fluctuation group [(16.35 ±2.45) pg/ml] were evidently higher than the other two groups (P ＜ 0.05).Conclusions Acute glucose fluctuations induced the enhancing of oxidative stress and expression of inflammatory cytokines in the aorta,which leads to start NF-κB signaling pathway.
