Study of the expression of Id1 gene to invasion of the pituitary adenoma

VernacularTitle:Id1基因表达与垂体腺瘤的侵袭性研究
Author: Bo MA ; Zhiqiang LI ; Zan SHEN
Publication Type:Journal Article
Keywords: Inhibitor of DNA binding 1 (Id1) gene; Pituitary adenoma; Invasion
From: Chinese Journal of Primary Medicine and Pharmacy 2015;22(1):1-4,后插1
CountryChina
Language:Chinese
Abstract: Objective To explore the pathogenesis of invasive pituitary adenomas,and to improve the effect of clinical treatment.Methods 15 pairs of invasive pituitary adenoma tissues and noninvasive pituitary adenoma tissues were selected from 114 specimens collected and the Id1 gene expression and Id1 protein expression in invasive pituitary adenoma tissues and noninvasive pituitary adenoma tissues were tested by quantitative RT-PCR,Western blot and immunohistochemical methods;Then small interfering RNA (siRNA) was used to interfer Id1 gene expression in NQ-04 cells.Results The Id1 gene expression in invasive pituitary adenoma tissues was significantly higher than that in noninvasive pituitary adenoma tissues(n =15,t =2.725,P =0.013) ;The migration of pituitary adenoma cells interfered was significantly reduced.Conclusion The invasive pituitary adenoma tissues has high level of Id1 gene expression,which may be correlated with its invasion.And Id1 is expected to become a biological marker of diagnosis and determination in the prognosis of pituitary adenoma.