Effects of triptolide on neutrophils asthmatic mice of WBC and EOS in bron-choalveolar lavage fluid
10.3969/j.issn.1000-484X.2015.07.009
- VernacularTitle:雷公藤甲素对中性粒细胞性哮喘小鼠肺泡灌洗液中 WBC 和 EOS 的影响
- Author:
Xiongbin LI
;
Mingdi CHEN
;
Junfen CHENG
;
Shenghui SUN
;
Yonglian LIN
;
Geyi WU
- Publication Type:Journal Article
- Keywords:
Triptolide;
Lipopolysaccharide;
Neutrophilic asthma;
Bronchoalveolar lavage fluid;
Switzerland staining
- From:
Chinese Journal of Immunology
2015;(7):906-908,911
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the influence of triptolide on neutrophils asthmatic mice of WBC and EOS in bronchoalveolar lavage fluid.Methods:Using ovalbumin ( OVA) combined with lipopolysaccharide ( LPS) method to establish sensitized asthmatic mice,BALB/c mice were randomly divided into 32 neutrophilic asthma group ( NA group ) , neutrophil triptolide intervention group (TLN group),neutrophil dexamethasone group (DXN group) and normal control group (NC group),n=8,hemocytometer calculated for each group of mice bronchoalveolar lavage fluid ( BALF) of the total number of WBC and EOS;smears stained Switzerland View in-flammatory cell infiltration.Results: In bronchioalveolar lavage fluid, numbers and infiltrations of WBC and EOS were significantly decreased in the DXN,TLN group than those in the NA group(P<0.05);but were significantly higher than the NC group (P<0.05), the DXN group above parameters were significantly higher than the TLN group ( all P<0.05 ) .Conclusion: Triptolide can reduce the total number of BALF WBC and EOS,inhibit lung WBC,EOS infiltration,ease neutrophilic airway inflammation.
