Effect of lncRNA HOTTIP on proliferation, migration and invasion of cervical cancer cells
10.7644/j.issn.1674-9960.2015.06.008
- VernacularTitle:长链非编码RNA HOTTIP对宫颈癌细胞增殖、迁移及侵袭的影响
- Author:
Fang LIU
;
Lian LI
;
Huan ZHANG
;
Weiyi ZHANG
;
Xiaojie WU
;
Yongyi XI
;
Yanrong ZHOU
;
Hongxing CHEN
;
Yanli LIN
- Publication Type:Journal Article
- Keywords:
lncRNA;
HOTTIP;
uterine cervical neoplasms;
cell proliferation;
cell movement;
neoplasm invasiveness
- From:
Military Medical Sciences
2015;(6):443-447,452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the potential biological effect of long non-coding RNA( lncRNA) HOXA transcript at the distal tip( HOTTIP) on proliferation, migration and invasion of cervical cancer cells.Methods HOTTIP small interference RNA(siRNA) was transfected into HeLa and C-33A cervical cancer cell lines, with negative siRNA as a control.qPCR assay was performed to confirm the knock-down of the level of HOTTIP.CCK8 assay and colony-forming unit (CFU) assay were performed to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell proliferation.Wound healing assay was performed to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell proliferation and migration.Tumor invasion assay was used to evaluate the effect of HOTTIP knock-down on HeLa and C-33A cell invasion. Results The expression level of HOTTIP was efficiently knocked down by siRNA 48 h post transfection.The results of CCK8 assay and CFU assay showed that HOTTIP knock-down significantly decrease of cervical cancer cell proliferation. Wound healing assay result indicated that HOTTIP knock-down obviously suppressed cervical cancer cell proliferation and migration.Tumor invasion assay results demonstrated that HOTTIP knock-down significantly suppressed cervical cancer cell invasion.Conclusion HOTTIP levels in HeLa and C-33A cervical cancer cell lines can be efficiently knocked down with the siRNA strategy, and the HOTTIP knock-down can significantly suppress the tumor characteristics of cervical cancer cells, including the ability of proliferation, migration and invasion.
