Regulation of PPARβactivation on protein synthesis and IL-1βexpression in hypertrophic cardiomyocytes

VernacularTitle:PPARβ激动剂对肥大心肌细胞蛋白合成及IL-1β表达的影响
Author: Li SHENG ; Ping YE ; Chunguang HAN ; Yongxue LIU
Publication Type:Journal Article
Keywords: peroxisome proliferator-activated receptor; cardiac hypertrophy; interleukin-1β; angiotensinⅡ
From: Military Medical Sciences 2015;(6):453-455,479
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of activation of peroxisome proliferator-activated receptor β/δ ( PPARβ/δ)on protein synthesis and expression of angiotensin (Ang)Ⅱ-induced hypertrophic myocytes(MC) in vitro.Methods Hypertrophy in neonatal rat cardiac MC culture was established with AngⅡ, then the effect of GW0742 on hypertrophy was detected.The synthetic rate of protein in MC was detected by 3 H-leucine incorporation.mRNA and protein expression of atrial natriuretic IL-1βwas measured by reverse transcription-polymerase chain reaction ( RT-PCR) and Western-blotting. Results and Conclusion GW0742 could reduce the synthetic rate of protein in hypertrophic MC while down-regulating the mRNA and protein expression of IL-1β, but no changes were observed after treatment with DMSO.The result demonstrated that activation of PPAR beta/delta inhibited cardiac hypertrophy in vitro and this effect might be related to inflammatory factors.