T2 mapping and quantitative analysis of the porcine patellar osteoarthritis model in vitro by collagenase
10.3969/j.issn.1002-1671.2015.04.036
- VernacularTitle:胶原酶消化猪髌软骨模型T2 mapping 成像与定量分析
- Author:
Chaoxiang YANG
;
Wei WANG
;
Chan QUEENIE
;
Ling CHEN
;
Wen LIANG
;
Xianyue QUAN
- Publication Type:Journal Article
- Keywords:
magnetic resonance imaging;
articular cartilage;
osteoarthritis
- From:
Journal of Practical Radiology
2015;(4):667-670,677
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the ability of T2 mapping in early detection of the change of cartilage collagen by quantitative analysis.Methods 20 porcine patellae were randomly assigned into 4 groups,3 treated groups and 1 control group.The samples of 3 treated groups were respectively immersed in PBS with 100 mg/100 mL,1 50 mg/100 mL and 200 mg/100 mL typeⅡ collagenase for 4 h,whereas control group samples in PBS for 4 h.T2 relaxation times of superficial,deep and full layers of cartilage and mean densi-ty after Van Gieson stain were measured.The correlation of T2 relaxation times and mean density were statistically analyzed.Results Gross pathology showed all articular cartilages maintained intact after treatment.T2 relaxation times of superficial and full layers and mean density increased significantly in any treated group in comparison with control group(P <0.05).Significant differences in T2 relaxation times of superficial layers and mean density were found between any two treated groups,whereas no difference of T2 re-laxation time of full layers was found between 100 mg/100 mL and 1 50 mg/100 mL treated groups(P >0.05).Pearson correlation analysis showed a close correlation(r=-0.837)between T2 relaxation times of full layers and mean density.Conclusion T2 map-ping can adequately detect the change of cartilage collagen in the early stage of osteoarthritis,and shows a good clinical application prospect.
