Role of HIF-1αin quinolinic acid-induced injury in PC12 cells
10.3969/j.issn.1001-1978.2015.04.011
- VernacularTitle:低氧诱导因子-1α在喹啉酸诱导的PC12细胞损伤中的作用
- Author:
Yongjin LI
;
Kaiyong YANG
;
Yi ZHANG
;
Yuefang CHEN
;
Lirong DUAN
;
Xiaojia HUANG
- Publication Type:Journal Article
- Keywords:
quinolinic acid;
PC1 2 cells;
cell injury;
cell apoptosis;
HIF-1 α;
signaling pathway;
oxidative stress
- From:
Chinese Pharmacological Bulletin
2015;(4):493-498,499
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the role of HIF-1 αin PC1 2 cell injury induced by quinolinic acid.Methods PC1 2 cells were treated with quinolinic acid at the do-ses of 2.5,5 and 1 0 mmol·L -1 ,the cell viability was determined by MTT reduction assay and LDH as-say,the intracellular levels of oxygen species was measured by assessing SOD and MDA levels,cell ap-optosis was determined by Hoechst 33258 staining,the intracellular distribution of HIF-1 αwas examined by HIF-1 αimmunostaining,and the expressions of HIF-1 α,Akt,p-Akt,Bcl-2 and Bax were determined by im-munoblotting analysis.Results Quinolinic acid in-duced cell injury in PC1 2 cells in a dose-dependent manner,and potentiated oxygen radical production and cell apoptosis.In addition,quinolinic acid enhanced HIF-1 αexpression and accumulation in nuclei.The p-Akt expression and Bax/Bcl-2 ratio was increased by quinolinc acid in PC1 2 cells.Conclusions HIF-1 αand Akt mediate qunolinc acid-induced cell apoptosis in PC1 2 cells.And cellular oxidative stress may con-tribute to the injury as well.
