HO-1 expression in adipose tissue of obese young SD rats and its role in inflammation and anti-inflammatory mechanism
10.11958/j.issn.0253-9896.2015.04.009
- VernacularTitle:肥胖SD幼鼠脂肪组织血红素加氧酶-1表达与炎症状态和抗炎机制的探讨
- Author:
Tingting LIU
;
Geli LIU
;
Jing ZHAO
;
Juan HE
;
Pengli BAO
;
Xiaoming DING
- Publication Type:Journal Article
- Keywords:
heme oxygenase (decyclizing);
adipose tissue;
macrophages;
obesity;
rats,Sprague-Dawley
- From:
Tianjin Medical Journal
2015;(4):367-369,452
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the change of HO-1 expression in adipose tissue of obese young SD rats as well as its relationship with macrophage infiltration and polarization. Methods Three-week old SD rats (n=24) were randomly divided into 2 groups, routine diet group (NC) and high fat diet group (FC). After feeding 4 weeks, triglyceride (TG), high den?sity lipoprotein (HDL-C), fasting glucose and insulin were compared between these two groups and the insulin resistance in?dex was calculated. The gene expressions of HO-1, IL-6, IL-10 and MCP-1 were assessed by quantitative PCR. Infiltration and polarization of macrophages and M2 macrophages in the visceral adipose tissue were examined by immunohistochemis?try. Results The levels of FINS, FBG and HOMA-IR in rats of FC group were higher than those of rats in NC group after 4 weeks feeding (P<0.05). The level of HO-1, IL-6, MCP-1 in rats from FC group were significantly higher while level of IL-10 were lower compared with those in rats from NC group after 4 weeks of feeding (P<0.05). In samples from FC groups, more macrophages were detected in adipose tissue by DAB staining than those from NC group. There was no significant dif?ference (P>0.05) in MOD value of F4/80 and CD206 between these two groups (P>0.05). Conclusion The infiltration of macrophage in visceral adipose tissue of obese young SD rats significantly increased while HO-1 expression was reactively increased. This insinuated that HO-1 might play an important role in anti-inflammatory mechanism through regulating polar?ization of macrophages.
