Effect of bone marrow-derived mesenchymal stem cells on fracture healing in an ovariectomized rat model
10.3969/j.issn.1673-4130.2015.14.012
- VernacularTitle:大鼠骨髓间充质干细胞体外分离及对原发性骨质疏松性骨折的实验研究
- Author:
Peng LI
;
Chengshuo HUANG
;
Xiang GAO
;
Guangmou CHEN
;
Zhijun LIU
;
Rong ZENG
;
Jiaqi CHU
- Publication Type:Journal Article
- Keywords:
Bone mesenchymal stem cells;
Osteoporosis;
Fracture
- From:
International Journal of Laboratory Medicine
2015;(14):1984-1985,1987
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the therapeutic effect of bone marrow‐derived mesenchymal stem cells (BMSCs) on osteoporotic fracture healing .Methods Bone marrow of the rat bilateral femur and tibia bone tissue were collected ,and BMSCs were isolated by the whole bone marrow adherence method .Sixty female 11‐week‐old SD rats were ovariectomized (OVX) to induce osteoporosis followed by bilateral fracture generation .Twenty rats were left without giving any further treatment (OVX controls) ,20 received injection of saline (OVX+placebo control) and 20 were given injection of BMSCs (OVX+BMSCs) .X‐ray scan was performed at 3‐day ,4‐week and 8‐week post‐fracture ,respectively .Results Flow cytometry analysis revealed that the isolated BMSCs express sur‐face antigens similar to those reported previously .X‐ray results showed that compared with OVX and OVX+placebo groups ,BM‐SCs treatment markedly accelerated fracture healing of in osteoporotic rats .Conclusion Transplantation of BMSCs can effectively improve the healing of primary osteoporotic fracture .
