The Immunohistochemical analysis for the expression of survivin, an inhibitor of apoptosis protein, in non-small cell lung cancer.
10.4046/trd.2000.48.6.909
- Author:
Mi Hye KO
;
Na Hye MYOUNG
;
Jae Whan LEE
;
Eun Mi CHO
;
Jae Seuk PARK
;
Keun Youl KIM
;
Kye Young LEE
- Publication Type:Original Article
- Keywords:
Survivin;
Apoptosis;
Immunohistochemistry;
Lung Cancer;
IAP;
p53
- MeSH:
Adult;
Apoptosis;
Blotting, Western;
Carcinogenesis;
Carcinoma, Non-Small-Cell Lung*;
Cell Line;
Horseradish Peroxidase;
Humans;
Immunohistochemistry;
Inhibitor of Apoptosis Proteins*;
Lung Neoplasms;
Neoplasm Metastasis;
Prognosis;
Recurrence
- From:Tuberculosis and Respiratory Diseases
2000;48(6):909-921
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Defects in apoptotic signaling pathways play important role in tumor initiation, progression, metastasis and resistance to treatment. Several proteins which may promote tumorigenesis by inhibiting apoptosis were identified. The survivin protein is the member of inhibitor of apoptosis protein(IAPs) family which inhibits apoptosis. Unlike other IAPs, it is expressed in during the fetal period but not in adult differentiated tissues. Many reports have stated that survivin is selectively expressed in many cancer cell lines and cancer tissues. We performed immunohistochemical analysis for survivin expression in non-small cell lung cancer to get evaluate its clinical implication. METHODS: Twenty nine surgically resected lung cancers were examined. Immunohistochemical staining were performed by immuno-peroxidase technique using avidin-biotinylated horseradish peroxidase complex in the formalin-fixed, paraffin-embedded tissue 4 µm section. Anti-survivin polyclonal antibody was used for primary antibody and anti-p53 monoclonal antibody was also used to analyze the correlation between survivin and p53 expression. The survivin expression scores were determined by as the sum of the stained area and intensity. RESULTS: Immunohistochemical analysis showed cancer specific expression of survivin in 20 of 29 cases (69.0%). Western blot analysis also showed the selective survivin expression in turmor tissue. There was no correlation between survivin expression and clinicopathological parameters and prognosis. We analyzed the correlated between survivin expression and p53 expression, but found none. CONCLUSION: We confirmed the tumor specific expression of survivin in non-small cell lung cancer But this pression was not correlated with clinical parameters as well as histlogy, tumor stage recurrence, and sur rate. Also it ws not statistically correlated with the expression of p53.
