Optimization of stability preservation conditions for N-terminal pro-brain natriuretic peptide
10.3969/j.issn.1000-484X.2015.04.017
- VernacularTitle:N端前脑钠肽稳定保存体系的优化
- Author:
Cui WANG
;
Yunlong WANG
;
Liang MING
;
Peiguo ZHENG
;
Nanchang XIE
;
Yulin LI
;
Jichuang WANG
- Publication Type:Journal Article
- Keywords:
N terminal brain natriuretic peptide;
Serum;
Stability
- From:
Chinese Journal of Immunology
2015;(4):517-521,526
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the best preservation conditions of N-terminal pro-brain natriuretic peptide( NT-proBNP) and provide detection references with stable performance for detection kits.Methods: ELISA was used to quantitatively detect the changes in NT-proBNP contents in various preservation solutions.The effects of basic buffer system, preservative Proclin300 and antibiotics on the preservation of NT-proBNP were analyzed using univariate analysis.The combination of various factors was then optimized using orthogonal experiments, to identify the best preservation system for NT-proBNP.Results: The univariate analysis determined that the basic buffer system for NT-proBNP was 0.02 mol/L phosphate buffered saline(PBS) at pH7.2,the addition of pre-servative Proclin300 could extend the preservation time of NT-proBNP at 37℃ by one day, the combined addition of penicillin and streptomycin prolonged the preservation time of NT-proBNP by one day compared with individually adding penicillin or streptomycin.The orthogonal experiments identified a preservation solution for NT-proBNP as 20%calf serum,1/1 000 Proclin300,120 U/ml penicillin and 80 U/ml streptomycin in a basic buffer system of 0.02 mol/L PBS at pH7.2.This solution was used to preserve an NT-proBNP reference sample at 37℃.Seven days later,the calibrated fixed-value of the sample at 37℃was only 1.3%lower than that at 4℃.Conclusion:Optimized NT-proBNP serum preservation solution could preserve NT-proBNP standard sample at 37℃ for seven days.
