Expression of IL-21 in mice with viral myocarditis and relationship with level of adenine nucleotide translocator antibody
10.3969/j.issn.1000-484X.2015.04.004
- VernacularTitle:白细胞介素21在小鼠病毒性心肌炎中的表达及其与抗ANT抗体的相关性
- Author:
Fan YANG
;
Hong WANG
;
Baoping TAN
;
Hao WU
- Publication Type:Journal Article
- Keywords:
Myocarditis;
Interleukin 21;
Adenine nucleotide translocator antibody
- From:
Chinese Journal of Immunology
2015;(4):453-455,461
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To explore the expression of IL-21 and the relationship with adenine nucleotide translocator antibody, ( ANT antibody ) in different courses of viral myocarditis ( VMC ) mice, and to explore the role and significance of IL-21 in VMC.Methods:To induced VMC mice model, BALB/c male mice were peritoneally injected ( IP ) with Coxsackievirus B3, mice peritoneally injected with PBS were taken as the controls.On 0,1,2,3,4 and 6 w after IP,the level of IL-21 mRNA in mice myocardic tissue was determined by real-time RT-PCR.The expression of IL-21 protein and ANT antibody in serum was evaluated by EILSA.Results:Comparing with the controls,the expression of IL-21 mRNA and protein increased from 1st week after IP,reaching the peak on the 2nd week,and maintaining a higher trend until 6th week.All the results at each time except that of 0 week were higher than those of controls(P<0.05).The levels of ANT antibody were also increased from 1st to 6th week,reaching the peak on the 2nd week all the results at each time except that of 0 week were higher than those of controls(P<0.05).Pearson analysis showed that there was rela-tionship between IL-21 and ANT antibody( r=0.88,P<0.01).Conclusion:The datas show that local significantly increased levels of IL-21 in myocardium and serum, and have positive relationship with the level of adenine nucleotide translocator antibody, which indicates that IL-21 may play a role in the pathogenesis of mice virus myocarditis.
