Effects of miR-30 a on cell cycle and dendritic cell-suppressive function of MSCs
10.3969/j.issn.1000-484X.2015.04.006
- VernacularTitle:miR－30a影响MSCs的细胞周期及其对DCs的抑制作用
- Author:
Erling HU
;
Dan LIU
;
Huishuang MIAO
;
Yahong HUANG
;
Yayi HOU
- Publication Type:Journal Article
- Keywords:
miR-30a;
Mesenchymal stem cells(MSCs);
Cell cycle;
Dendritic cells(DCs)
- From:
Chinese Journal of Immunology
2015;(4):462-466,471
- CountryChina
- Language:Chinese
-
Abstract:
Objective:Mesenchymal stem cells( MSCs) have self-renewal capacity and potential to differentiate into the cells.It was reported that the expression of miR-30a changed in some immune diseases.But it remains unclear the effect of miR-30a on the im-munoregulatory functions of MSCs.Here we studied the impact of miR-30a on the phenotype,cell viability,apoptosis,cell cycle and im-munoregulatory functions of MSCs.Methods: The mixed enzyme methods were used for the isolation of human umbilical cord MSCs.Flow cytometry(FCM)was used to investigate the effect of overexpressed miR-30a on the phenotype of MSCs.CCK-8 was used to examine the cell viability of miR-30a-overexpressed MSCs.Annexin V/PI was used for the detection of apoptosis of MSCs.Q-PCR and Western blot were used to investigate the effect of miR-30a on the expression of Cyclin E2( CCNE2).CCNE2 was one putative target of miR-30a predicted by Targetscan database.The effects of miR-30a-overexpressed MSCs on the maturation of dendritic cells(DCs)were determined.Results:Overexpression of miR-30a blocked the cell cycle of MSCs in the G0/G1 phase by inhibiting the expression of CCNE2,but did not affect the phenotype, cell viability and apoptosis of MSCs.When co-cultured with DCs, although MSCs down-regulated the expression of CD40 and CD86 on DCs,overexpression of miR-30a more significantly enhanced the suppressive impact of MSCs on the maturation of DCs.Conclusion: miR-30a affects the cell cycle of MSCs and enhances its immunosuppressive effect on DCs.
