Knockout gata4 gene and establish ment of a zebrafish model of congenital heart disease by TALEN
10.3969/j.issn.1671.7856.2015.004.001
- VernacularTitle:应用 TALEN 技术敲除 gata4基因建立斑马鱼先天性心脏病模型
- Author:
Jing LIU
;
Jialing HE
;
Guo BAO
;
Nan LI
;
Tianqi WANG
;
Changyong ZHANG
;
Deming SUN
- Publication Type:Journal Article
- Keywords:
TALEN;
gata4 gene;
Zebrafish;
Model,animal;
Congenital heart disease
- From:
Chinese Journal of Comparative Medicine
2015;(4):1-7,13
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a gata4 gene knockout zebrafish model of congenital heart disease, and construct transcription activator-like effector nuclease ( TALEN) vectors targeting gata4 gene.Method We construct TALEN vectors targeting zabrafish gata4 gene using unit assembly method and the in vitro-transcribed TALEN mRNAs were microinjected into one-cell stage zebrafish embryos.The efficiency of TALEN was identified by injected embryos, and mutations of zebrafish were screened and confirmed the different types through PCR and enzyme digestion.Results We successfully constructed correct targeting vectors by enzyme digestion and sequencing, and the gene knockout efficiency was 35.18%.We screened the mutant zebrafish and confirmed different types of gata4 gene mutations.Conclusions A gata4 knockout zebrafish model is successfully established, it can provide a good animal model for further research of congenital heart diseases.
