Influence of olfactory bulbectomy on mouse neurobehaviors,hippocampal neurotransmission and depression relevant gene expressions
10.3969/j.issn.1001-1978.2015.05.019
- VernacularTitle:双侧嗅球切除对小鼠行为、海马神经递质及抑郁相关基因表达的影响
- Author:
Tingting WANG
;
Fei HUANG
;
Hui WU
;
Beibei ZHANG
;
Xincheng ZHANG
;
Xiaojun WU
;
Zhibi HU
- Publication Type:Journal Article
- Keywords:
depression;
olfactory bulb;
neurotransmit-ter;
hippocampus;
neurotrophic factor;
inflammatory factor;
antidepressant
- From:
Chinese Pharmacological Bulletin
2015;(5):686-690,691
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of behav-iors,neurotransmitters and depression related gene ex-pressions in hippocampus of C57BL/6 mice after olfac-tory bulbectomy (OB).Methods Forced swimming test (FST),tail suspension test (TST),open filed test (OFT),and elevated plus maze test (EPMT) were used to evaluate the behavioral changes 18 days after surgery. LC-MS/MS method was employed to measure the hippocampal neurotransmitters.Quantita-tive PCR approach was established to determine the hippocampal gene expressions associated with depres-sion.Results OB mice were hyperactive in OFT (P<0.05 ) accompanied with increased time spent in open arm of EPMT (P<0.01).Meanwhile,the sur-gery led to the reduction of the ratio of 5-HIAA/5-HT (P <0.01 ) but the increase of Glu/GABA (P <0.01 ) in hippocampus.Moreover,OB elevated the gene expressions of BDNF,Trkb,GDNF,CD1 1 b and TNF-αbut down-regulated that of TPH2 in hippocam-pus (P<0.05 ).Conclusion Behavioral alternation of OB mice was a result of comprehensive effect of the changes of neurotransmission and depression related genes,which call us special attention in using OB as an animal model for research and development of anti-depressants.
