Effects and mechanisms of endurance exercise for 8 weeks on contractile function and mitochondrial biogenesis in rat skeletal muscles
10.3969/j.issn.1001-1978.2015.05.020
- VernacularTitle:耐力运动8周对大鼠骨骼肌收缩功能和线粒体生物合成的影响及机制
- Author:
Ni QIU
;
Cong LI
;
Weijin FANG
;
Xuemei WEI
;
Yulian HE
;
Yan XIONG
- Publication Type:Journal Article
- Keywords:
endurance exercise;
soleus;
extensor digi-torum longus;
skeletal muscle contractility;
mitochon-drial biosynthesis;
rat model
- From:
Chinese Pharmacological Bulletin
2015;(5):691-696,697
- CountryChina
- Language:Chinese
-
Abstract:
Aim This study was aimed to explore the influence and mechanism of the long-term exercise on skeletal muscle contraction and mitochondrial biosyn-thesis in different muscle fibers.Methods Soleus (SOL)and extensor digitorum longus (EDL)were i-solated from SD male rats with platform running train-ing for eight weeks.The changes of contractility under different electrical stimulation were observed, mito-chondrial biosynthesis,including ATP content,mito-chondrial DNA,the gene expression of PGC-1αand NRF were also detected.Results Long-term endur-ance exercise can improve twitch tension and titanic tension of SOL and EDL ,but only enhanced the fa-tigue resistance in SOL.ATP contents were significant-ly increased in the two types of muscles,but mtDNA content,PGC-1αexpression and NRF translation were only obviously enhanced in SOL,in accompanied with an increase in p-AMPK/AMPK protein ratio.Conclu-sion Long-term endurance exercise increased skeletal muscle contractility and improved the anti-fatigue abili-ty in SOL,which may be associated with increase in mitochondrial biosynthesis via activated AMPK-PGC-1αaxis.
