Study on biocompatibility of Ad-rhBMP-2 transfection on rabbit BMSCs combined with allogeneic DBM
10.3969/j.issn.1671-8348.2015.10.001
- VernacularTitle:腺病毒 rhBMP-2转染兔 BMSCs 复合同种异体脱钙骨基质的生物相容性研究
- Author:
Weiliang CAI
;
Qiang LI
;
Yinkuan NING
;
Chengcong WU
;
Jiabin CHEN
;
Zhengsong SHI
- Publication Type:Journal Article
- Keywords:
bone morphogenetic proteins;
adenoviridae;
bone marrow;
mesenchymal stem cells;
decalcified bone matrix
- From:
Chongqing Medicine
2015;(10):1297-1299,1304
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the biocompatibility of rabbit bone marrow mesenchymal stem cells (BMSCs)combined with allogeneic decalcified bone matrix(DBM)after transfecting adenoviral recombinant human bone morphogenetic protein-2(Ad-rhBMP-2).Methods The rabbit allogeneic DBM material was prepared according to the Ursit method.After transfecting Ad-BMP-2 on rabbit bone marrow mesenchymal stem cells,the immunohistochemical was used to detect the expression of BMP-2 in the transfected cells;after 48 h of transfection,the cells were planted on the allograft DBM,then the scanning electron microscopy was used to observe the cell growth and adhesion condition on material,and the proliferation condition of BMSCs was detected by MTT. Results After 48 h of adenoviral transfection,BMSCs could express BMP-2 successfully.The scanning electron microscopy showed that the cells after transfection adhered well and massively proliferated on DBM material.The MTT assay showed that the prolifer-ation condition of the cells after transfection planted on DBM was normal,which showed no statistically significant difference when compared with the control group (P >0.05).Conclusion The Ad-BMP-2 transfection on BMSCs is well biocompatible to allogene-ic DBM.
