Detection of EWSR1 gene translocation in paraffin-embedded Ewing family tumor by fluores-cence in situ hybridization
10.13315/j.cnki.cjcep.2015.03.015
- VernacularTitle:荧光原位杂交检测 EWSR1基因易位在尤因肉瘤家族肿瘤疑难病例诊断中的应用
- Author:
Xiao LI
;
Chong LIU
;
Guoxin SONG
;
Guoqiang PING
;
Qixing GONG
- Publication Type:Journal Article
- Keywords:
Ewing sarcoma;
fluorescence in situ hybridization;
EWSR1 gene
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(3):302-305,306
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To evaluate the application of fluorescence in situ hybridization ( FISH) in detection of EWSR1 gene translocation in formalin-fixed paraffin-embedded tissue samples of Ewing family tumor ( EFT) . Methods Four cases of EFT were collected and their clinical pathological features were obsered. Interphase FISH was carried out in paraffin-embedded tissue of EFT cases, using a EWSR1 dual colar break-apart probe. Fifteen cases of other soft tissue tumor were selected as negative control. Results Translocation of EWSR1 was detected in four EFT cases, which may be a adjuvantly diagnositic value for the diagnosis of atypical cases. Conclu-sions FISH may serve as an adjunctive diagnostic tool in problematic cases of EFT, however, FISH results should be interpreted con-cerning clinical pathological features, morphological patterns and immunohistological results.
