Effects of Xianfu Wenyang Tongluo Drink on ANCA Associated Antigen in Thromboangiitis Obliterans Rat Models
10.3969/j.issn.1005-5304.2015.06.023
- VernacularTitle:仙附温阳通络饮对血栓闭塞性脉管炎模型大鼠抗中性粒细胞抗体相关抗原的影响
- Author:
Jing ZHAO
;
Xuejun WANG
;
Rui BEN
;
Shuming ZHANG
;
Huaijun LI
- Publication Type:Journal Article
- Keywords:
Xianfu Wenyang Tongluo Drink;
thromboangiitis obliterans;
ANCA;
MPO;
PR3;
LAMP-2;
humoral immunity;
rats
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2015;(6):80-82,83
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of Xianfu Wenyang Tongluo Drink (XFWYTLD) on ANCA associated antigen in rats with thromboangiitis obliterans;To discuss its mechanism. Methods Totally 72 SPF male Wistar rats were randomized into sham-operation group, model group, Mailuoning Granule group and XFWYTLD low, medium, and high dose groups. Method of femoral artery injecting sodium laurate was used to duplicate models. From the second day after modeling, the rats in sham-operation group and model group were fed with distilled water, while other groups received gavage with relevant medicine. 15 days later, the activity of MPO in serum was detected through the method of ultraviolet spectrophotometry;the protein expressions of PR3 and LAMP-2 in femoral artery and the surrounding tissues were detected through the method of immunohistochemisty. Results Compared with sham-operation group, the activity of MPO in serum and the protein expressions of PR3 and LAMP-2 in rats of model group were significantly higher;Compared with model group, the activity of MPO in serum and the protein expressions of PR3 and LAMP-2 in rats of all medication administration groups decreased, among which the XFWYTLD medium dose group showed the most obvious decrease. Conclusion XFWYTLD may lower levels of ANCA associated antigen, and further inhibit humoral immune function.
