Inhibitory effect of ophiopogonin-B on adhesion, invasion and migration of A549 cells in vitro
10.3969/j.issn.1001-1978.2015.05.014
- VernacularTitle:麦冬皂苷B对A549细胞株体外黏附、侵袭及迁移的抑制作用及机制研究
- Author:
Meijuan CHEN
;
Ruolin ZHAO
;
Yuanyuan GUO
;
Limin NING
;
Xu ZHANG
- Publication Type:Journal Article
- Keywords:
ophiopogonin-B;
non-small cell lung canc-er;
adhesion;
invasion;
migration;
matrix metallopro-teinase-2;
matrix metalloproteinase-9
- From:
Chinese Pharmacological Bulletin
2015;(5):660-663,664
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the inhibitory effects of ophiopogonin-B (OP-B ) on cell adhesion,invasion and migration in non-small cell lung cancer (NSCLC) A549 cells in vitro and its possible mechanism.Meth-ods Cell adhesion assay and transwell chamber assay were used to detect the ability of cell adhesion,migra-tion and invasion.qRT-PCR was used to detect the mRNA levels of MMP-2 and 9 .Meanwhile ,Western blot assay was performed to determine the protein levels of MMP-2/9 and p-Akt.Results OP-B significantly inhibited the ability of cell adhesion,invasion and mi-gration in A549 cells at the concentration of 10 μmol· L-1 (P<0.01 ).Meanwhile,it inhibited the mRNA and protein levels of MMP-2 and MMP-9 and down-regulated the phosphorylation of Akt (P <0.0 1 ). Conclusion OP-B inhibits cell adhesion,invasion and migration in A549 cells through down-regulation of the mRNA and protein expression of MMP-2/9 ,and the inhibitory effect on the expression of p-Akt.
