Expression of miR-339-5 p in diffuse large B-cell lymphoma and its significance
10.13315/j.cnki.cjcep.2015.04.012
- VernacularTitle:弥漫大B细胞淋巴瘤中miR-339-5 p的表达及意义
- Author:
Yiyao TU
;
Guihong ZHANG
;
Kaiyang DING
;
Zhengsheng WU
;
Zhaohui WANG
;
Feng YANG
- Publication Type:Journal Article
- Keywords:
lymphoma;
diffuse large B cell;
miR-339-5p;
Ki-67;
BCL-6
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(4):409-412,417
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the relevance between the expression of miR-339-5p and the clinicopathological characteristics in diffuse large B-cell lymphoma (DLBCL). Methods The level of miR-339-5p expression was detected in 123 cases of diffuse large B-cell lymphoma tissues and 20 cases of reactive lymphoid hyperplasia tissues by chromogenic in situ hybridization ( CISH) technique. The expression of Ki-67 and BCL-6 protein was examined in diffuse large B-cell lymphoma tissues by immunohistochemical technique (IHC) (EnVision two-steps), and the correlation between the expression of miR-339-5p and BCL-6 and the clinicopathological param-eters was also analyzed. Results The positive rates of miR-339-5p were 39. 8% (49/123) in DLBCL tissues, which was significantly lower than that in RH tissues (90%, 18/20). The positive rates of miR-339-5p were 31. 0% (22/71) for germinal center B-cell-like (ABC type) DLBCL, which was significantly lower than that in activated B-cell-like (GCB type) DLBCL (27/52, 51. 9%). The low-er expression of miR-339-5p in DLBCL was correlated with late Ann Arbor staging and high-risk group of international prognostic index (P<0. 05). The survival rates of miR-339-5p negative patients of ABC type and GCB type of DLBCL were significantly lower than that of the positive patients (P<0. 01). The levels of miR-339-5p expression in DLBCL were negatively correlated with the levels of BCL-6 expression in DLBCL (P<0. 01). Conclusion The low expression of miR-339-5p might be relatived with the progression and poor prognosis of DLBCL.
