Mechanisms of tumor cell’ s apoptosis triggered by gold nanorods
- VernacularTitle:纳米尺度金棒诱导肿瘤细胞凋亡的机制研究
- Author:
Fulei ZHANG
;
Cheng JIANG
;
Changhong KE
;
Tai YU
;
Sufen LI
;
Shangjing GUO
;
Wei LI
- Publication Type:Journal Article
- Keywords:
gold nanorod;
mitochondria;
apoptosis
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(4):42-44,47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanism that gold nanorods trigger apoptosis in cancer cells.Methods Gold nanorods was synthesized by gold seed growing method, and its characterization was detected; gold nanorods on cell proliferation-toxicity were evaluated by CCK-8 Kit and apoptosis were detected by flow; mitochondrial membrane potential were tested by JC-1 and activation of Caspase 9 and Caspase 3 were detected by western blot. Results The results found that gold nanorods had nontoxic to normal cells, but highly toxic to tumor cells; and with the increasing of gold nanorods’ working time, the percentage of apoptotic cancer cells was increasing; in addition to, normal cells’ mitochondrial membrane potential did not change, but cancer cells had a significant reduction in mitochondrial membrane potential.Conclusion This study proves that gold nanorods induce apoptosis through the mitochondrial apoptosis pathway.
