Correlation of EGFR gene mutation with its amplification and protein expression in non-small cell lung cancer
10.13315/j.cnki.cjcep.2015.06.014
- VernacularTitle:非小细胞肺癌EGFR基因突变与扩增及蛋白表达的相关性分析
- Author:
Shunping CHEN
;
Haiyan SU
;
Wenqiao WU
;
Yinghao YU
;
Wei LIU
;
Hongwu SHEN
- Publication Type:Journal Article
- Keywords:
1ung neop1asms;
non-sma11 ce11 1ung cancer;
epiderma1 growth factor receptor;
PCR;
FISH;
immunohistochemistry
- From:
Chinese Journal of Clinical and Experimental Pathology
2015;(6):657-661,665
- CountryChina
- Language:Chinese
-
Abstract:
Purpose To investigate the corre1ation of the epiderma1 growth factor receptor( EGFR)gene mutation and amp1ification and protein expression with occurrence and deve1opment of non-sma11 ce11 1ung cancer( NSCLC),and to exp1ore the re1ationship be-tween the mutation and amp1ification of EGFR gene and other c1inica1 patho1ogica1 parameters. Methods qRT-PCR,FISH and immu-nohistochemistry were used to detect EGFR gene(exons 18,19,20 and 21)mutation,amp1ification and protein expression in paraf-fin-embedded tissues of NSCLC. Results EGFR gene(exons 18,19,20 and 21)mutation rate was 58. 18%(32/55)in NSCLC with qRT-PCR techno1ogy,in which the occurrence rate of exon 19 de1etions and exon 21 mutation of L858R was 87. 50%(28/32). EGFR gene mutation rate was significant1y different in gender,smoking history and patho1ogica1 type(P<0. 01),but no statistica1 sig-nificance in age,1ymph node metastasis and TNM staging(P>0. 05). EGFR gene amp1ification rate was 23. 64%(13/55)and its protein expression rate was 70. 91%(39/55). Both EGFR gene mutation and amp1ification was c1ose1y corre1ated(P<0. 05),but the two states of EGFR gene and its protein expression had no corre1ation(P>0. 05). Conclusion EGFR gene mutation with high pro-tein expression of NSCLC is common1y found in fema1e,no-smoking and adenocarinoma patients,who are main candidates of a tyrosine kinase inhibitor( TKI)screening. EGFR gene mutation and amp1ification is typica11y corre1ated,but their consequence is unknown, which needs to be further investigated. EGFR gene mutation and amp1ification is not consistent with protein expression,its under1ying machanism is to be determined.
