Expression of IL-12 family subunit genes in mice C6 glioma cells
10.3969/j.issn.1000-484X.2015.05.005
- VernacularTitle:IL-12家族亚基基因在大鼠C6胶质瘤细胞表达研究
- Author:
Jin SUN
;
Xiaoning LIN
;
Junqing ZHANG
;
Yanlin HUANG
;
Xinhua TIAN
- Publication Type:Journal Article
- Keywords:
IL-12 family;
Mice C6 glioma cells;
Real-time quantitative PCR;
Tumor immunology
- From:
Chinese Journal of Immunology
2015;(5):600-602,606
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the expression of IL-12 family subunit genes by real-time quantitative PCR in mice C6 glioma cells,construct the basis of the brain glioma research on IL-12 family in the future.Methods:Mice C6 glioma RNA was abstracted and reversed transcription cDNA.The mice C6 glioma cells mRNA expression influence of IL-12 family subunit genes was compared and analyzed by real-time quantitative PCR.Results: In mice C6 glioma cells, high expression abundances in IL-23a, IL-12a, midlde expression abundances in EBI3, IL-27, low expression abundance in IL-12b.Conclusion: IL-12 families are closely related to the occurrence and development of glioma,IL-12,IL-23 are regarded as the most potential anti-glioma cytokines among them,research de-velopments will bring a new way of brain glioma immune therapy.
